Fig. 3
Comparison of key proteoform-specific aggregation kinetics between PD and MSA. a Protein aggregation rate (PAR) across all regions. PAR was defined as the inverse of the lag phase (T−1), which is the time required to reach the amplification threshold (set to 103.5 r.f.u). Red data points denote individual MSA-P cases, and navy data points denote individual MSA-C cases. b Maximum relative fluorescence across all regions. Maximum relative fluorescence was calculated as the maximum/minimum fluorescence ratio. c Gradient of amplification across all regions. The gradient of amplification was determined by calculating the gradient of the tangent at the inflection point of each amplification curve. d–f Comparison of PAR (d), maximum relative fluorescence (e), and gradient of amplification (f) within specific brain regions (medulla, substantia nigra, and hippocampus). Filled circles (black) denote the mean kinetic values, the crossbars denote the median kinetic values, and the box plot extremities denote the interquartile range. ***P < 0.001, **P < 0.01, *P < 0.05; Welch’s t-test a–f, one-way ANOVA with Tukey’s multiple comparison adjustment used to compare between regions d–f